When you see your veterinarian, they will ask you for your dog’s medical history as well as any changes in behavior. A physical examination will be performed and your veterinarian will closely look at any masses or tumors present on the skin or subcutaneously. A biopsy will probably be performed. This generally requires that a needle be inserted into the mass and drawing out any fluid or cells. If the mass is hard and unable to be aspirated using a needle then a surgical biopsy may be necessary. The lymph nodes may also be needle aspirated for a biopsy.
Upon endocytic PRR binding, actin - myosin cytoskeletal rearrangement adjacent to the plasma membrane occurs in a way that endocytoses the plasma membrane containing the PRR-PAMP complex, and the microbe. Phosphatidylinositol and Vps34 - Vps15 - Beclin1 signalling pathways have been implicated to traffic the endocytosed phagosome to intracellular lysosomes , where fusion of the phagosome and the lysosome produces a phagolysosome. The reactive oxygen species , superoxides and hypochlorite bleach within the phagolysosomes then kill microbes inside the phagocyte.